Black sand nanoparticles and heat stress impacts the neurological and oxidative stress indices and splenic-renal histology of Clarias gariepinus

In Egypt, while many studies have focused on the radiometry and mineralogy of black sands, research on their effects on nearby aquatic organisms is rare. This study aimed to assess the combined effects of heat stress (HS) and black sand nanoparticles (BS-NPs) on renal function, antioxidant responses (TAC, SOD, CAT), neuro-stress indicators (AchE, cortisol), and to conduct histopathological investigations in the kidney and spleen tissues of African catfish Clarias gariepinus over a 15-day period to exposure to control, HS (32 °C), BS (6.4 g/kg diet) and HS + BS groups. The outcomes revealed that thermal stress alone showed no significant difference from the control. However, creatinine and uric acid levels were significantly higher in the BS-NPs and HS + BS-NPs groups (p < 0.001). Antioxidant markers (TAC, SOD, and CAT) were substantially reduced across all treated groups (0.05 ≥ p < 0.0001). AchE levels were significantly elevated in BS-NPs and HS + BS-NPs (p < 0.001), while cortisol levels were higher in these groups but not significantly different in HS. Degeneration and necrosis in the white and red pulps, scattered lymphocytes, and increased collagen fiber surrounding blood vessels and the lining of the ellipsoid structure were all evident in the spleen, along with the enlargement of the melanomacrophage centers with big granular, irregular, and brown pigments (hemosiderin). Our study, therefore, provides new insights into how heat stress, an abiotic environmental factor, influences the toxicity of black sand nanoparticles in catfish.

www.nature.com/scientificreports/ the potential welfare consequences of periodic warm water treatments on Atlantic salmon (Salmo salar) over an extended period of time.Under 25 °C (control) and 30 °C (experimental), hematological, biochemical, genotoxic, and histopathological indicators were assessed as biomarkers for heat stress in the neotropical catfish (Rhamdia quelen) 8 .Klein et al. 9 conducted a study to investigate the impact of increasing temperatures on the antioxidant defense system and oxidative stress indices in two Antarctic fish species, namely Notothenia coriiceps and Notothenia rossii.Furthermore, Schleger et al. 10 conducted an assessment of the antioxidant defenses in the freshwater fish Astyanax lacustris under conditions of heat stress.
The black sand is naturally occurring mineral depositions along the coastal areas, dominated by an iron titanates and minor oxides such as calcium, magnesium and silicon 11 .Other strategic and economic minerals like rutile, monazite, leucoxene, magnetite, garnet, and zircon were also present in these deposits.Black sand placer deposits are found in the form of sediments along beaches and coastal sand dunes.Numerous studies in radiometry and mineralogy have been conducted on these sands [12][13][14][15][16][17][18][19] .X-ray Diffraction (XRF) analysis of black sand collected from the Egyptian Red Sea coast revealed the presence of various oxides, such as Iron(III) oxide (Fe 2 O 3 ), Silicon dioxide (SiO 2 ), Titanium dioxide (TiO 2 ), Magnesium oxide (MgO), Calcium oxide (CaO), and Aluminum oxide (Al 2 O 3 ) 20 .Studies examining how black sand affects animals in the wild or through experiments are relatively uncommon.The impact of black sand, which was isolated from the beach of the Red Sea, on Oreochromis niloticus was assessed by Saad and Sayed 20 .More recently, Abdelbaki et al. 21investigated the structure, spreading, and occurrence of black sand along the Red Sea, Egypt, through a comprehensive field and lab investigations.
An expanding class of anthropogenic contaminants, nanoparticles interact with their surroundings in a variety of ways.In numerous industrial fields, including the manufacture of paints, personal care products, biological sensors, and electrical catalysts, NPs are extensively utilized 22 .NPs from industrial or domestic waste can alter the aquatic ecology and endanger aquatic life when they are released into the environment 23 .According to studies, Titanium dioxide nanoparticles (TiO 2 -NPs) may cause DNA damage, oxidative stress, inflammation, genotoxic response, and tissue distribution 24,25 .C. gariepinus showed changes in behavior, neurological and physiological markers after being exposed to Silicon dioxide nanoparticles (SiO 2 -NPs) nanoparticles 26 .Also, adult zebrafish (Danio rerio) that exposed to SiO 2 -NPs showed impaired reproductive performance and oxidative stress 27 .
It has been indicated that the combined influence of elevated temperatures and chemical pollution synergistically modifies aquatic ecosystems 28 .In this scenario, the consequences of elevated temperatures and the presence of carbon nanotubes on the biochemistry and physiology of Mytilus galloprovincialis were studied 29 .Also, Baag et al. 30 conducted an experimental evaluation to evaluate the combined effects of increased temperature and zinc oxide nanoparticles on the mud crab Scylla serrata.
The most significant species of fish used in aquaculture, the African catfish (Clarias gariepinus) is widely popular as a source of protein due to its low diseases mortality rate 31 .Utilizing histopathological indicators and evaluating biochemical markers are advantageous techniques for figuring out how animals respond to stressors 32 .
The individual and combined impacts of rising water temperature and BS-NPs on catfish C. gariepinus are poorly understood.Accordingly, the current work aimed to study the impact of water temperature increases and BS-NPs separately and in combination on measures of renal function, antioxidant responses, neuro-stress indicators, and histological examinations in C. gariepinus.

Sampling of the black sand (BS)
The BS samples were obtained from Hurghada city, the Red Sea beach, Egypt (S.D 1).The isolation, and separation of BS were done according to Saad and Sayed 20 .The nanoparticles were synthesized via top-down approach, in this method the BS-NPs sample was grinded using a ball-milling machine (Fritsch Mini-Mill pulverisette 23, GmbH, Duisburg, Germany) for 1 h.

Characterization of BS-NPs sample
A comprehensive analysis of the BS-NPs sample was investigated at Assiut University.X-ray fluorescence (XRF) at the Faculty of Engineering revealed their chemical composition.The morphology and particle size of the BS-NPs were examined utilizing transmission electron microscopy (TEM).Images were captured with a JEOL Model JSM-5400 LV (Joel, Tokyo, Japan) set to 80 kV.X-ray diffraction (XRD) using a Phillips PW 2103/00 diffractometer with Ni-filtered CuKα radiation was employed to determine the crystalline phases, nature, and crystal size.Finally, the functional groups present in the samples were identified using Fourier-transform infrared (FTIR) spectroscopy, employing a Nicolet 6700 spectrophotometer.

Fish collection and exposure conditions
Clarias gariepinus of good health, displaying no signs of infection, were procured from a local fish farmer.Catfish were (male and female) had an average weight of 200 ± 25 g and average length of 25 ± 5 cm.The experiments adhered to Test Guideline No. 203 Fish; Acute Toxicity Testing outlined by OECD 33 with slight modification.Prior to experimentation, catfish were acclimatized in 120 L containers for 15 days under controlled conditions at a temperature of 26 ± 2 °C, pH level range of 7.2-7.6,oxygen saturation at a minimum of 80%, and a natural photoperiod of 12 h of darkness followed by 12 h of light.Twice daily, the C. gariepinus were fed a commercial diet (SKRETTING, Egypt) containing 30% protein.The diet included ingredients such as soy meal, wheat bran, maize, crude protein, lipids, crude fibers, fish meal, calcium, and sodium chloride, following the guidelines set by the Organization for Economic Co-operation and Development (OECD) in 2019 33 .Maintaining good water quality, 40% of the water was changed daily to remove fish waste.

Statistical analysis
GraphPad Prism 9.0 (GraphPad Software Inc.) was used to statistically analyze all the data, and the results were shown as mean ± standard error of the mean (SEM).To see if there were any important differences between the control group and the other groups, a statistical test called one-way ANOVA was used.Then, another test, Fisher's LSD, to pinpoint exactly which groups were different from the control.Differences between treatments and controls were believed statistically significant at p ≤ 0.05.The asterisk superscript (*) was used graphically to indicate the significance level (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Characterization of BS NPs sample
The semi-quantitative chemical analysis of the BS-NPs sample was conducted by XRF.Analysis of the BS-NPs sample (Fig. 1a) revealed that Fe 2 O 3 makes up the largest portion (26.3%) followed by SiO 2 at 11.9%.TiO 2 , MgO, CaO, and Al 2 O 3 were also present in smaller amounts (around 3-10%).The remaining metal oxides were each less than 2% of the sample.Figure 1b shows the TEM micrograph of the dispersion of BS-NPs sample in ethanol deposited over a Cu grid.The BS-NPs sample consists of a dispersion of almost plate-like particles with an average particle size of 266 nm.The black color particles are attributed to a magnetite particle, while the gray particles are related to quartz.The XRD analysis of the BS-NPs sample is depicted in Fig. 1c 01-074-1867).Figure 1d shows the BS NPs sample's FTIR spectrum.The O-H stretching and bending modes of hydroxyl groups are responsible for the bands seen at 3397 and 1633 cm −1 , respectively.Furthermore, the symmetric and asymmetric vibrations of the C-H of saturated aliphatic compounds are responsible for the bands that were identified at 2926 and 2855 cm −1 , respectively.

Kidney functions
The resulting data of kidney function measured in the serum of C. gariepinus are graphically presented as means ± SEM (Fig. 2A,B).Compared to control fish; the mean value of creatinine scored from BS-NPs and HS + BS-NPs was significantly higher (p < 0.001).Even though the mean creatine value measured in HS exposed group was higher than the control one, it was not statistically significant.As well as creatinine, the mean value of uric acid measure in BS-NPs and HS + BS-NPs was higher (p < 0.001) compared to the control.Uric acid measurements in the HS group were very close to control values (p > 0.05).

Antioxidant parameters
The effects of BS-NPs and HS on the antioxidant indicators (TAC, SOD, and CAT) in Clarias gariepinus is shown in Fig. 2 (0.05 ≥ p < 0.0001).TAC was recorded from the BS-NPs, HS, and their combination (HS + BS-NPs) showed the highest levels of significance compared to the control group.Similarly, the average SOD data from each treatment showed a comparable decline trend when compared to the control group (p < 0.001) (Fig. 2C).On the other hand, the heat stress had a greater impact on the mean TAC than SOD (Fig. 2D,E), with the latter showing a higher BS-NPs induced drop.www.nature.com/scientificreports/

Neurological parameters
The AchE activity in the HS group was not significantly different from the control group (p > 0.05).In contrast, the AchE activity in the BS-NPs group and the HS + BS-NPs group was significantly different from the control group (p < 0.001), indicating a distinct divergence from the control values (Fig. 3).Conversely, cortisol levels rose in both the BS-NPs and HS + BS-NPs groups relative to the control fish.Nevertheless, there was no statistically significant difference in the mean cortisol levels between the HS and the control group (p > 0.05).

Kidney
Results of histological analysis of the kidney of C. gariepinus exposed to thermal stress (32 °C), BS-NPs (9.6 g BS/ kg diet), and a combination of both treatments for 15 days are presented in Fig. 4A-D.The kidneys in the control group have normal positions for glomeruli, Bowman's capsules, tubular lumen, blood arteries, hemopoietic  www.nature.com/scientificreports/tissue, and intra-renal spaces (Fig. 4A).Different degrees of damage, such as dissociation in the renal tubules, hypertrophy and degeneration in the glomerulus, degeneration in the collecting tubules, and proliferation in the hemopoietic tissue, have been seen in fish exposed to heat stress (Fig. 4B).Moreover, BS-NPs group revealed adverse impacts on the kidney including various degrees of injuries ranging from dissociation in the renal tubules, shrinkage of the tubular lumen, shrinkage of glomerulus and increasing in the Bowman's space, proliferation in the haemopoietic tissue and degeneration and necrosis of renal tubules (Fig. 4C).The combined treatments caused significant kidney damage, including disrupted architecture, tubular hypertrophy, and glomerular shrinkage, along with increased Bowman's space and necrosis of collecting tubules (Fig. 4D).PAS-technique exhibited positive reactivity in brush border, the basement membrane of the renal tubules and the glomerulus of the control fish (Fig. 5A).Thermal stress-exposed fish revealed moderate decline in polysaccharide materials in brush border, the basement membrane of the renal tubules, and glomerulus (Fig. 5B).PAS-technique in BS-NPs exposed fish revealed more depletion in polysaccharide materials in brush border, the basement membrane of the renal tubules, glomerulus and hemopoietic tissue compared to those of control ones (Fig. 5C).Fish exposure to a combination of both treatments, showing great depletion in the polysaccharide materials in comparison to those of control ones and the other treatments (Fig. 5D).Sections of kidney in all experimental groups stained with Sirius red stain for demonstration of connective tissues collagenous fiber.The control group showed normal distribution of collagen in renal capsule and the wall of blood vessels (Fig. 6A).Thermal stress-exposed fish revealed this density of staining were increased around blood vessels and slightly faint in renal capsule when compared with the control (Fig. 6B).BS-NPs exposed fish displayed increased collagen intensity of staining in blood vessels, glomerulus and renal corpuscle as compared with the control and the thermal stress group (Fig. 6C).Fish exposure to a combination of both treatments revealed more collagen fiber around blood vessels and glomerulus compared to those of the control ones and the other treatments (Fig. 6D).www.nature.com/scientificreports/Spleen Sections of C. gariepinus spleen stained with control and exposed to thermal stress (32 °C), BS-NPs (6.4 g BS/ kg diet), and a combination of both treatments are presented in Fig. 7A-D.The control group showed morphology of spleen represented in both red and white pulps were clearly observed, increase in both lymphocytes aggregated in the white pulp and ellipsoid bodies which located in the core of the white pulp.While red pulp full of hemopoietic tissues, especially red blood cells (RBCs) (Fig. 7A).In contrast, there were a variety of alterations in the thermal stress group, including an increase in the size of the white pulp that contained newly produced lymphocytes and an unclear separation between the white and the red pulps.However, expansion of the melanomacrophage centers with large granular, irregular, and brown pigments (hemosiderin).Deformation in the lining (cubic epithelium) of ellipsoid structures which presented as terminal capillaries in both white and red pulps which surrounded by a sheath of acidophilic fibrous connective tissues and dispersed lymphocytes.Shrunken red pulp which contains dispersed hematopoietic tissues (Fig. 7B).In BS-NPs group, red pulp increased in size containing hemopoietic tissues mainly RBCs.The boundary between white and red pulps were not clear in comparison with the control group.Shrinkage and necrosis in the white pulp containing a few newly formed lymphocytes.Melanomacrophage centers with large, irregular, black and brown pigments (melanin and hemosiderin) were noticed.Ellipsoid structures were not observed in both white and red pulps (Fig. 7C).Combined group showed degeneration and necrosis in white and red pulps and dispersed lymphocytes.Boundary between red and white pulps were not observed, also increased in size and number of the melanomacrophage centers with large, irregular, black and brown pigments (melanin and hemosiderin).Vascular dilatation of blood vessels full of blood corpuscles and ellipsoid structures were not observed in both white and red pulps (Fig. 7D).PAStechnique for all experimental groups is shown in Fig. 8A-D.In the control group, positive reactivity in white and red pulps and ellipsoid structure were detected (Fig. 8A).Thermal stress-exposed fish revealed moderate decline in polysaccharide materials in both white, red pulps and ellipsoid structure (Fig. 8B).PAS-technique in the BS-NPs exposed fish revealed more depletion in polysaccharide materials in splenic tissues as compared to those of control ones (Fig. 8C).Fish exposure to a combination of both treatments, showing great depletion in the polysaccharide materials compared to those of control ones and other treatments (Fig. 8D).Sections of spleen in all experimental groups stained with Sirius red stain for demonstration of connective tissues collagenous fiber (Fig. 9A-D).In the control group showed normal distribution of the collagen in lining of ellipsoid structure and blood vessels (Fig. 9A).Thermal stress and BS-NPs exposed fish revealed increased density of staining around blood vessels and in lining of ellipsoid structure as compared with the control group (Fig. 9B,C).While in the combined group, revealed more collagen fiber around blood vessels and lining of ellipsoid structure compared to the control and the other treatments (Fig. 9D).

Discussion
Fe 2 O 3 (26.3%),SiO 2 (11.9%),TiO 2 (9.72%),MgO (3.74%), CaO (3.29%), and Al 2 O 3 (2.96%) are the principal oxides identified in the BS-NPs sample.whereas the remaining metal oxides that have been found have levels of less than 2%.This result is consistent with that obtained by Saad and Sayed 20 .The TEM micrograph of the BS-NPs showed plate-like particles with an average particle size of 266 nm.The black color particles are attributed to a magnetite particle, while the gray particles are related to quartz as stated by Li et al. 47 and Prasdiantika et al. 48.
The XRD pattern of the BS-NPs sample showed several diffraction peaks.The analysis confirms that the main components of this sample are iron compounds, along with silicate (containing silicon), titanate (containing titanium), and calcite (calcium carbonate) 49 .This composition is consistent with the results obtained from the XRF analysis earlier.The average crystallite size for the BS-NPs sample was evaluated utilizing Scherrer equation 21,[50][51][52] and found to be equal to 18.6 nm.The O-H stretching and bending modes of hydroxyl groups are responsible for the bands seen in the FTIR spectrum of the BS-NPs sample at 3397 and 1633 cm −1 , respectively 53 .Furthermore, the symmetric and asymmetric vibrations of the C-H of saturated aliphatic compounds are responsible for the bands that were identified at 2926 and 2855 cm -1 , respectively.Asymmetric stretching of C-O, stretching vibrations of Si-O-Si, Fe-O bond, Fe-O stretching, Fe-O stretching or Ti-O bond vibration, and Si-O bending are the explanations given for the bands found at 1472, 1008, 874, 534, 456, and 418 cm -1 , respectively.Knowing the content of BS-NPs is crucial for accurately interpreting their biological effects, assessing safety, and understanding the mechanisms through which they interact with biological tissues 49 .The elemental composition and structural properties of these nanoparticles determine how they behave in biological systems, influence their toxicity, and provide insights into their potential applications 54 .Diseases like lung cancer, pancreatic, liver, and kidney have been associated with a variety of sources of radioactive materials released from the terrestrial radionuclides 238 U, 226 Ra, 232 Th, and 40 K, as well as radon gas and its daughters and ingesting radioactive food 55,56 .Radioactive BS was discovered in the Rosetta coastal zone during an aerial gamma ray spectrometry investigation carried out in 2003 by the Nuclear Material Authority of Egypt 19 .The main source of radioactive enrichment 57 was the monazite-rich BS beach on Penang Island, Malaysia.Beach sand samples collected on the Mediterranean coast of Egypt, specifically from Baltim beaches in Kafr El-Sheikh governorate, were found to contain naturally occurring radioactive isotopes, including 226 Ra, 232 Th, and 40 K 16 .
Studies comparing different sands for solar stills have shown that BS absorbs heat much better than yellow sand, making it more efficient 58 .Similarly, research by Omara and Kabeel 59 found black sand to be the most efficient heat absorber among various sands tested in solar stills.This suggests that BS is probably going to contribute to warming and heat stress in nearby aquatic and terrestrial environments.
The long-term effects of human-caused climate change have drawn more attention to the implications of temperature variation for ecology and evolution.Regarding temperature-dependent chemical toxicity in freshwater fish, research has shown that the median lethal concentration (LC 50 ) for ten chemicals exhibits an inverted V-shaped distribution along a temperature gradient 6 .Fish lack the ability to regulate their body temperature, making them susceptible to temperature increases 60 .Consistent to this, male sexual characteristics appear to be more susceptible to continuous warming, as evidenced by the shorter length and slower growth of Poecilia reticulata sperm exposed to temperature fluctuations 5 .Thermal delousing, which uses heated water to remove salmon lice, has grown in the salmon industry since 2015 61 .There's a significant knowledge gap regarding the impact of thermal delousing on salmon health and welfare.Additionally, the long-term consequences after treatment have not been extensively studied 62 .Investigating internal tissues and other indicators after stress is therefore crucial.For example, kidney was identified as a critical accumulator tissue because of their great accumulative www.nature.com/scientificreports/potential towards different NPs 63 .The present study has been supported by previous investigations that evaluated an elevation in kidney biomarkers following exposure to heat stress and nanomaterials.Similar to the current study, Dawood et al. 64 reported a significant effect of heat stress (32 °C) on C. gariepinus urea and creatinine.The plasma concentrations of creatinine and uric acid in O. niloticus increased significantly upon exposure to aluminum oxide nanoparticles 65 .Ali et al. 66 revealed elevation in urea and creatinine Rattus norvegicus exposed to silica-based nanoparticles.Alterations in renal blood flow, renal function, or production of urine can all contribute to a rise in creatinine 67 and the histological changes in kidneys that are currently being detected may support this. C. gariepinus and O. niloticus showed higher urea levels after being exposed to atrazine, mancozeb, chlorpyrifos, lambda-cyhalothrin, and their combination 68 .The current findings agree with Mahmoud et al. 69 , they concluded that the concentrations of creatinine and uric acid exhibited different significant alteration in C. gariepinus treated with silver NPs.Together, the increased levels of creatinine and uric acid as well as the histological deterioration imply that BS and heat may be detrimental to catfish.Furthermore, in the current research, temperature had a significant impact on the antioxidant parameters both alone and in conjunction with BS NPs, demonstrating the combined effects of thermal stress.Consistent with our findings, research investigating the impact of nanomaterials on fish antioxidants has revealed a downward trend in the antioxidant enzyme levels SOD, CAT and TAC.The current findings may corroborate recently published research showing that temperature fluctuations and photoperiod variations have a significant effect on the activity of antioxidant enzymes in Atlantic salmon 70 .The reason antioxidants respond to heat stress is most likely due to the fact that, at a certain temperature, cellular metabolic activity increases until it reaches a threshold, beyond which the metabolic rate decreases 71 .Also, under heat stress, substantial changes in serum, lysozyme, aminotransferase, liver SOD, GPX, and malondialdehyde levels were resulted in salmon 72 .Because they catalyzed the superoxide dismutation reaction, SOD are considered essential components of the first stage of the enzymatic antioxidative defense mechanism 73 .Similarly, Waheed et al. 74 observed a noteworthy reduction in antioxidant enzymes SOD and CAT, in O. niloticus subjected to mercury chloride combined with heat stress.
In addition to kidney and oxidative biomarkers, cortisol is released in response to stress, which alters the neuroendocrine system and causes a number of physiological and metabolic processes that facilitate the tissues' www.nature.com/scientificreports/easy absorption of glucose and lactate 75 .Moreover, extreme suppression of AchE activity disrupts nervous system function since AchE can provide important information about neuronal activities essential for survival and performance 76 .In accordance, AchE activities in Mytilus galloprovincialis, showed significant changes in behavior and oxidative status following the exposure to nanoparticles and phosphonates 22 .Likewise, D. rerio subjected to SiO 2 -NPs showed notable deficits in the AchE and the cortisol 27 .Histologically, triple-stained sections of the kidney and spleen employed in this work demonstrated the combined effects of heat and BS stress 77 .The present work, along with histopathological investigations carried out by other authors, has yielded a beneficial approach such neurological and oxidative stress for assessing the adverse effects related to heat stress nanomaterials 78 .The BS NPs-induced histopathological alterations observed here agree with previous works conducted on different stressors and vertebrate species including fish 78 .For example, in catfish Mystus vittatus exposed to ZnS NPs 79 , C. gariepinus exposes to silver NPs 80 , Rattus norvegicus exposed to silica NPs 81 polystyrene NPs-exposed Hypophthalmichthys molitrix 82 .Comparable to the current findings Monfared et al. 83 reported shrinkage in glomerular diameter and formation of intra cytoplasmic vacuoles in the kidney of Oncorhynchus mykiss subjected to silver NPs.Copper NPs was reported to had an impact on the histological structure of liver and kidney in Cyprinus carpio 84 .
TiO 2 NPs increased gill histopathological damages in C. carpio when combined with Ag NPs 85 .This demonstrates the detrimental effect of TiO 2 and others (e.g., SiO 2 ) analyzed in the current investigation and supports our suggestion that BS NPs are deposited in tissues.Supporting study 86 has demonstrated that O. niloticus exhibited tissue (ranked as: spleen > intestine > kidney > liver > gills > brain > muscles) accumulation following exposure to Fe 2 O 3 NPs.TiO 2 NPs have been demonstrated to be immunotoxic to fish and to lessen fish neutrophils capacity against bacteria 87 .In aquatic environments variations in temperature and acidity affect the toxicity and bioavailability of some pollutants, such heavy metals and alter the biological processes.The amplified negative chemical effects brought on by the high temperature are probably linked to the disruption in oxygen consumption and aerobic scope 88 .
Histopathological changes in the gills, such as aneurysm hemorrhage, epithelial cell hypertrophy, hyperplasia, and skin ulcers, were linked to the treatment against salmon lice, which involved raising the temperature of the farmed fish to 34 °C for 30 s 62 .In line with the present investigation, Vicentini et al. 8 found that catfish R. quelen displayed a number of sublethal changes at temperature 30 °C, including changes to their hematological system, activation of their antioxidant system, and modifications to their energy metabolism.
Interestingly, changes in kidney function and oxidation indicators, imply that the kidney and spleen degenerative lesions are most likely mediated by the oxidative stress brought on by the tissues-deposited NPs 24 .These combined changes appear to indicate the production of peroxides, free radicals, and reactive oxygen species (ROS), which are linked to cellular damage and lipid peroxidation 89 .In addition to kidney deterioration, the current study also showed several splenic degenerations, including necrotic white and red pulps, hemorrhage blood vessel dilatation, a decrease in polysaccharide materials, aggregated collagen fiber around blood vessels, and ellipsoid structure lining.These findings are consistent with earlier research on stressors and spleen-mediated toxicities.For example, O. niloticus exposed to iron oxide NPs 86 , C. gariepinus exposed to Thiamethoxam 90 , seabream Sparus aurata exposed to zinc oxide particles 91 , carp exposed to Avermectin 92 , zebrafish exposed to acrylamide 93 and (Di(2-ethylhexyl) phthalate) 94 .The occurrence of oxidative stress, inflammation, and apoptosis is typically strongly linked to splenic injury 92 .
Consequently, even though there are many applications for nanoparticles, their chemical stability and ability to exist on a variety of surfaces and forms helps amplifies their presence and transformation in ecosystems 95 .According to the current findings, along with previous works, temperature and chemical exposure have a complex mixed effect on aquatic species.Our histology findings on catfish are consistent with those of Saad and Sayed 20 on O. niloticus subjected to 15 days of BS.Their findings indicated that the liver, intestines, and brain exhibit various alterations including cellular degeneration, vacuolation, infiltration, hemorrhage congested blood vessels, etc.
The study findings of Abdelbaki et al. 21provided useful knowledges into the geological diversity of BS occurrences along the Red Sea, they underlined the need for additional research and evaluation.In accordance, the current study provided a sizable dataset about the ecotoxicological effects and potential impact of black sand on the aquatic ecosystems and associated fauna.
Future directions could involve studying the long-term and chronic effects of BS-NPs on fishes, exploring different concentrations and environmental interactions, and using advanced molecular techniques for deeper mechanistic insights.Expanding the range of biomarkers and refining the nanoparticle synthesis process could also enhance the study's relevance and applicability in real-world scenarios.

Conclusion
The heat stress and BS-NPs caused different degrees of damage to the kidney and spleen, in addition to altered kidney, oxidative, and neurological signs.According to the chemical structure, BS contains a few substances that have been linked to the harm of aquatic life and associated organisms.These findings suggest that, in addition to the changes in temperature brought on by global warming, BS presents an increasing risk to the adjacent aquatic and terrestrial environments.The combined stresses of pollution exposure and climate change may be detrimental for biota that are already on the border of their physiological tolerance limit.Accordingly, further research is still required to make sure that nanomaterials will not have any harmful effects before we use them in industrial household products and public health-related settings.
Asymmetric stretching of C-O, stretching vibrations of Si-O-Si, Fe-O bond, Fe-O stretching, Fe-O stretching or Ti-O bond vibration, and Si-O bending are the explanations given for the bands found at 1472, 1008, 874, 534, 456, and 418 cm −1 , respectively.

Fig. 1 .
Fig. 1.Illustrates the comprehensive characterization of BS-NPs sample.(a) Shows the elemental composition of the BS-NPs as determined by X-ray fluorescence (XRF), indicating the presence and abundance of key elements.(b) Presents a transmission electron microscopy (TEM) image revealing the morphology and size distribution of the nanoparticles.(c) Displays the X-ray diffraction (XRD) pattern, which provides insights into the crystalline structure of the BS-NPs.(d) Depicts the Fourier-transform infrared (FTIR) spectrum, identifying the functional groups associated with the nanoparticles.

Fig. 4 .
Fig. 4. Transverse sections of control and treated fish kidney (H&E X 400).(A) Sections of control fish kidney showing the renal corpuscle (RC), renal tubules (RT), collecting tubules (CT) and haemopoietic tissue (HT).(B) Sections of fish kidney exposure to thermal stress (32C) showing dissociation in renal tubules (DIRT), Hypertrophy in the glomerulus (HYT), degeneration in collecting tubules (DCT) and degeneration in renal corpuscle (DRC).(C) Sections of fish kidney exposure to BS nanoparticles (9.6 g BS/kg diet) showing necrosis (N) and degeneration (D), shrinkage of the tubular lumen (TL), shrinkage of glomerulus (SHG) and increasing in the Bowman's space (BS).(D) Sections of fish kidney exposure to combination of both treatments showing dissociation (DIRT) and hypertrophy (HYT) in the renal tubules, shrinkage of the tubular lumen (TL), degeneration in the renal corpuscle (D), shrinkage of glomerulus (SHG) and increasing in the Bowman's space (BS).

Fig. 5 .
Fig. 5. Transverse sections of control and treated fish kidney (PAS X 400).(A) Sections of control fish kidney showing positive PAS reactivity in brush border (BB) and the basement membrane (BM) of the renal tubules.(B) Sections of fish kidney exposure to thermal stress showing moderate decline in polysaccharide materials in brush border (BB), the basement membrane of the renal tubules (BM) and glomerulus (G).(C) Sections of fish kidney exposure to BS nanoparticles showing more depletion in polysaccharide materials in brush border (BB), the basement membrane (BM) of the renal tubules, glomerulus (G) and hemopoietic tissue (HT).(D) Sections of fish kidney exposure to combination of both treatments showing great depletion in the polysaccharide materials in compared to those of control ones and other treatments.

Fig. 6 .
Fig. 6.Sections of control and treated fish kidney (Sirius X 400).(A) Sections of control fish kidney showing normal distribution of collagen in renal capsule (RC) and the wall of blood vessels (BV).(B) Sections of fish kidney exposed to thermal stress revealed density of staining were increased around blood vessels and slightly faint in renal capsule.(C) Sections of fish kidney exposed to BS nanoparticles showing increased collagen intensity of staining in blood vessels, glomerulus, and renal corpuscle.(D) Sections of fish kidney exposed to combination of both treatments showing more collagen fiber around blood vessels and glomerulus.

Fig. 7 .
Fig. 7.Sections of control and treated fish spleen (H&E X 400).(A) Sections of control fish spleen showing red (RP) and white pulps (WP), ellipsoid shape (Es) and lymphocyte (LYC).(B) Sections of fish spleen exposed to thermal stress (32C) showing increased in size in white pulp (WP), expansion of the melanomacrophage centers (Mm).(C) Sections of fish spleen exposed to BS NPs (9.6 g BS/kg diet) showing necrosis (N) in the white pulp.(D) Sections of fish spleen exposed to combination of both treatments showing necrosis (N) in white and red pulps, dilatation of blood vessel (DBV) and hemorrhage (Hr).

Fig. 8 .
Fig. 8. Transverse sections of control and treated fish spleen (PAS X 400).(A) Sections of control fish spleen exhibited positive reactivity in white and red pulps and ellipsoid structure.(B) Sections of fish spleen exposed to thermal stress showing moderate decline in polysaccharide materials in both white and red pulps also ellipsoid structure.(C) Sections of fish spleen exposed to BS nanoparticles showing more depletion in polysaccharide materials in splenic tissues.(D) Sections of fish spleen exposed to combination of both treatments showing great depletion in the polysaccharide materials.

Fig. 9 .
Fig. 9.Sections of control and treated fish spleen (Sirius X 400).(A) Sections of control fish spleen showing normal distribution of collagen in lining of ellipsoid structure and blood vessels.(B, C) Sections of fish spleen exposed to thermal stress and black sand nanoparticles showing increased density of staining around blood vessels and in lining of ellipsoid structure.(D) Sections of fish spleen exposed to combination of both treatments showing more collagen fiber around blood vessels and lining of ellipsoid structure.